This study describes comparative disposition of long acting moxifloxacin in goats and sheep after intravenous administration in five animals. Long acting moxifloxacin solution (10 % moxifloxacin in solution with L- arginine, N-butyl alcohol and benzyl alcohol) was injected in jugular vein. Blood samples were collected from contra lateral jugular vein in test tubes containing 30-50 IU heparin (anticoagulant) periodically from 0.083 to 72 h of drug administration. Drug concentrations in plasma were determined using High Performance Liquid Chromatography (HPLC) with Fluorescence Detector. The mobile phase consisted of a mixture of buffer (10 gm of tetrabutyl ammonium hydrogen sulphate per liter-deionised water) and acetonitrile (80: 20). The buffer was 0.067 M of disodium hydrogen phosphate with pH of 7.5. The flow rate was 1 ml.min-1 at ambient temperature. The effluent was monitored at 296 nm excitation and 504 nm emissions wavelength. HPLC with Fluorescence Detector method for plasma moxifloxacin assay was standardized with specific modification for plasma of goats and sheep in present study. Long acting moxifloxacin the plasma concentration of 0.001±0.000 µg.ml-1 and 0.016±0.001 µg.ml-1 after single dose intravenous and was maintained for up to 72 h in goats and sheep, respectively.
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